Recent Publications
Lijun Zhang, Xi'e Wang, Xia Peng, Yingjuan Wei, Rui Cao, Zhen Liu, Jixian Xiong, Xingfeng Ying, Ping Chen, and Songping Liang. 2007. Immunoaffinity Purification of Plasma Membrane with Secondary Antibody Superparamagnetic Beads for Proteomic Analysis. Journal of Proteome Research 6, 34 - 43.

Lijun. Zhang, Xi' e Wang, Xie Peng, Yingjuan. Wei, Rui Cao, Zhen Liu, Jixian. Xiong, Xingfeng. Ying, Ping Chen, Songping Liang. 2006. Proteomic analysis of low-abundant integral plasma membrane proteins based on gels. Cellular and Molecular Life Sciences 63(15),1790-1804.

Jian Zhou, Tieyang Zhou, Rui Cao, Zhen Liu, Jianying Shen, Ping Chen, Xianchun Wang, Song- ping Liang. 2006. Evaluation of the Application of Sodium Deoxycholate to Proteomic Analysis of Rat Hippocampal Plasma Membrane. Journal of Proteome Research 5, 2547- 2553.

Rui Cao, Xuanwen Li,Zhen Liu, Xia Peng, Weijun Hu , Xianchun Wang, Ping Chen, Jingun Xie, Songping Liang (2006) Integration of a Two-Phase Partition Method into Proteomics Research on Rat Liver Plasma Membrane Proteins Journal Proteome Research in press

Ping Chen, Xuanwen Li, Yalan Sun, Zhen Liu, Rui Cao, Quanyuan He, Meichi Wang, Jixian Xiong, Jinyun Xie, Xianchun Wang, Songping Liang. 2006. Proteomic analysis of rat hippocampal plasma membrane: characterization of potential neuronal- specific plasma membrane proteins. Journal of Neurochemistry 98 , 1126C1140.

Liu, Z.; Dai, J.; Dai, L.; Deng, M.; Hu, Z.; Hu, W.; Liang, S. Function and solution structure of Huwentoxin-X, a specific blocker of N-type calcium channels, from the Chinese bird spider Ornithoctonus huwena. J Biol Chem 2006, 281, (13), 8628-35.

Quan-Yuan He, Xiang-Hua Liu, Qiang Li, David J. Studholme, Xuan-Wen Li, Song-Ping Liang. 2006. G8: a novel domain associated with polycystic kidney disease and non-syndromic hearing loss. Bioinformatics 22(18), 2189-2191.

Lijun Zhang, Xin Liu, Jian Zhang, Rui Cao, Ying Lin, Jinyun Xie, Ping Chen, Yi Sun, Daiqiang Li, Songping Liang. 2005. Proteome analysis of combined effects of androgen and estrogen on the mouse mammary gland. Proteomics 6(2), 487-497.

Xiao, Y.; Tang, J.; Hu, W.; Xie, J.; Maertens, C.; Tytgat, J.; Liang, S. 2005. Jingzhaotoxin-I, a novel spider neurotoxin preferentially inhibiting cardiac sodium channel inactivation. J Biol Chem 280, (13), 12069-76.

Quan-Yuan He, Jing Cao, Xiang-Hua Liu, Miao-Xin Li, Yi-Song Liu, Jin-Yun Xie and Song-Ping Liang (2005) DEPD: a novel database for differentially expressed proteins bioinformatics 21(18) 3694-3696

Chen, J. Q.; Zhang, Y. Q.; Dai, J.; Luo, Z. M.; Liang, S. P. 2005. Antinociceptive effects of intrathecally administered huwentoxin-I, a selective N-type calcium channel blocker, in the formalin test in conscious rats. Toxicon 45, (1), 15-20.

Lijun Zhang, Jinyun Xie, Rui Cao Xi ' e Wang, Xiaohui Liu, Weijun Hu, Song Nie, Chunming Fan and Songping Liang (2005) Proteomic analysis of mouse liver plasma membrane: use of differential extraction to enrich hydrophobic membrane proteins Proteomics in press  (Attatchment)

Xiongzhi Zheng, Qi Zhu and Songping Liang. 2005.Sequence-specific assignment of 1H-NMR resonance and determination of the secondary structure of Jingzhaotoxin-I. Acta Biochimica et Biophysica Sinica 37(8):567-572.

Huang L Y, Li B X, Luo C, Xie J Y, Chen P, Liang S P(2004) Proteome comparative analysis of gynogenetic haploid and diploid embryos of goldfish( Carassius auratus ). Proteomics 3 (6) 245-251

Xianghua Liu, Quanyuan He, David J. Studholme, Qi Wu, Songping Liang and Long Yu (2004) NCD3G: a novel nine-cysteine domain in family 3 GPCRs. Trends Biochem. Sci. 29, 458–461 (Erratum)

Liang, S. 2004. An overview of peptide toxins from the venom of the Chinese bird spider Selenocosmia huwena Wang [=Ornithoctonus huwena (Wang)]. Toxicon 43, (5), 575-85.

Li, D.; Xiao, Y.; Xu, X.; Xiong, X.; Lu, S.; Liu, Z.; Zhu, Q.; Wang, M.; Gu, X.; Liang, S. 2004. Structure--activity relationships of hainantoxin-IV and structure determination of active and inactive sodium channel blockers. J Biol Chem 279, (36), 37734-40.

Ping Chen, Song Nie, Wei Mi, Xian-Chun Wang, Song-Ping Liang. 2004. De novo sequencing of tryptic peptides sulfonated by 4-sulfophenyl isothiocyanate for unambiguous protein identification using post-source decay matrix-assisted laser desorption/ionization mass spectrometry. Rapid Communications in Mass Spectrometry, 18(2):191-8.

Yucheng Xiao, Jianzhou Tang, Yuejun Yang, Meichi Wang, Weijun Hu, Jinyun Xie, Xiongzhi Zeng, and Songping Liang(2004) Jingzhaotoxin-III, a novel spider toxin inhibiting activation of voltage-gated sodium channel in rat cardiac myocytes. J Biol Chem. Jun 18;279(25):26220-6

Xiao, Y.; Liang, S. 2003. Inhibition of neuronal tetrodotoxin-sensitive Na+ channels by two spider toxins: hainantoxin-III and hainantoxin-IV. Eur J Pharmacol 477, (1), 1-7.

Liu, Z.; Dai, J.; Chen, Z.; Hu, W.; Xiao, Y.; Liang, S. Isolation and characterization of hainantoxin-IV, a novel antagonist of tetrodotoxin-sensitive sodium channels from the Chinese bird spider Selenocosmia hainana. Cell Mol Life Sci 2003, 60, (5), 972-8.

Jianbo Diao, Ying Lin, Jianzhou Tang, Songping Liang (2003) cDNA sequence analysis of seven peptide toxins from the spider Selenocosmia huwena Toxicon Vol. 42,715–723

Peng-Fei Zhang, Ping Chen, Wei-Jun Hu, Song-Ping Liang (2003) Huwentoxin-V, a novel insecticidal peptide toxin from the spider Selenocosmia huwena, and a natural mutant of the toxin: indicates the key amino acid residues related to the biological activity Toxicon 42,15–20

Liao Zhi, Dai Shu-Jia, Liu Xiu-Wen, Tang Zhong-Ming, Liang Song-Ping (2003) Pharmacokinetics of [125I] Huwentoxin-1 after epidural and intravenous administration to rhesus monkeys Chinese Pharmacological Bulletin 19(8) 944-949

Dongling Lia, Yucheng Xiao, Weijun Hu, Jinyun Xie, Frank Bosmans, Jan Tytgat, Songping Liang (2003) Function and solution structure of hainantoxin-I, a novel insect sodium channel inhibitor from the Chinese bird spider Selenocosmia hainana FEBS Letters 555, 616-622

Xiao Yu-Cheng, Liang Song-Ping (2003) Inhibition of Sodium Channels in Rat Dorsal Root Ganglion Neurons by Hainantoxin-IV, a Novel Spider Toxin Acia Biochemica of Biophysica SINICA 35(1):82-85

Yu-Cheng Xiao, Song-Ping Liang (2003) Purification and characterization of Hainantoxin-V, a tetrodotoxin-sensitive sodium channel inhibitor from the venom of the spider Selenocosmia hainana Toxicon 41 651–656

Xiong-Zhi Zeng, Qiao-Bin Xiao, Song-Ping Liang (2003) Purification and characterization of raventoxin-I and raventoxin-III, two neurotoxic peptides from the venom of the spider Macrothele raveni Toxicon 41:651–656

Yucheng Xiao, Songping Liang (2003) Inhibition of neuronal tetrodotoxin-sensitive Na+ channels by two spider toxins: hainantoxin-III and hainantoxin-IV European Journal of Pharmacology 477 1– 7

Qi Zhu, Songping Liang, Loı¨c Martin, Sylvaine Gasparini, Andre´ Me´nez, and Claudio Vita (2002) Role of Disulfide Bonds in Folding and Activity of Leiurotoxin I: Just Two Disulfides Suffice Biochemistry, 41, 11488-11494

Qin Shu, Shan-Yun Lu, Xiao-Cheng Gu, and Song-Ping Liang (2002) The structure of spider toxin huwentoxin-II with unique disulfide linkage: Evidence for structural evolution Protein Science , 11:245–252.

Kuan Peng, Qin Shu, Zhonghua Liu, and Songping Liang (2002) Function and Solution Structure of Huwentoxin-IV, a Potent Neuronal Tetrodotoxin (TTX)-sensitive Sodium Channel Antagonist from Chinese Bird Spider Selenocosmia huwena Journal Bio. Chem Vol. 277, No. 49, 47564–47571

Kuan Peng, Xiang-Ding Chen, Song-Ping Liang (2001) The effect of Huwentoxin-I on Ca2+ channels in differentiated NG108-15 cells, a patch-clamp study. Toxicon 39 (2001) 491-498

Qin Shu, Renhuai Huang and Songping Liang (2001) Assignment of the disulfide bonds of huwentoxin-II by Edman degradation sequencing and stepwise thiol modification Eur. J. Biochem. 268, 2301-2307

Yu Liu, Songping Liang. 2001. Chemical carboxyl-terminal sequence analysis of peptides and proteins using tribenzylsilyl isothiocyanate. Journal of Protein Chemistry 20(7), 535-541.

Songping Liang, Xiaolan Li, Menglin Cao, Jinyun Xie, Ping Chen, and Renhuai Huang (2000) Indentification of Venom Proteins of Spider S. huwena on Two-Dimensional Electrophoresis Gel by N-Terminal Microsequencing and Mass Spectrometric Peptide Mapping Journal of Protein Chemistry, Vol. 19, No. 3

Song-Ping Liang, Xiang-Ding Chen, Qin Shu,Yongqun Zhang, Kuan Peng (2000) The presynaptic activity of huwentoxin-I, a neurotoxin from the venom of the Chinese bird spider Selenocosmia huwena Toxicon 38:1237-1246

Min Li, Lu-Yi Li, Xiushan Wu, Song-Ping Liang (2000) Cloning and functional expression of a synthetic gene encoding huwentoxin-I, a neurotoxin from the Chinese bird spider (Selenocosmia huwena ) Toxicon 38 153-162

Feng Li, Songping Liang (1999) Assignment of the three disulfide bonds of Selenocosmia huwena
lectin-I from the venom of spider Selenocosmia huwena
Peptides 20:1027–1034

Shanyun Lu, Songping Liang,and Xiaocheng Gu (1999) Three-Dimensional Structure of elenocosmia huwena Lectin-I (SHL-I) from the Venom of the Spider Selenocosmia huwena by 2D-NMR Journal of Protein Chemistry, Vol. 18, No. 5, 509-617

Liang, S.; Shu, Q.; Wang, X.; Zong, X. Oxidative folding of reduced and denatured huwentoxin-I. J Protein Chem 1999, 18, (6), 619-25.

Qu, Y.; Liang, S.; Ding, J.; Ma Li.; Zhang, R.; Gu, X. 1999. Proton nuclear magnetic resonance studies on huwentoxin-I from the venom of the spider Selenocosmia huwena : 1. sequence-specific 1 H-NMR assignments. J Protein Chem 14,7.

Qi Shu, Song-Ping Liang (1998) Purification and characterization of huwentoxin-II, a neurotoxic peptide from the venom of the Chinese bird spider Selenocosmia huwena J. Peptide Res. 53, 486-491

Pei-Ai Zhou, Xian-Jie Xie, Man Li,Dong-Mei Yang, Zuo-Ping Xie, Xiang Zong and Song-Ping Liang (1997) Blockade of Neuromuscular Transmission By Huwentoxin-I, Purified from the venom of the Chinese Bird Spider Selenocosmia Huwena Toxicon. Vol. 35. No I, pp. 39-45

Liang,SP., Xia Zanxian and Xie Jinyun. 1997. Solid-phase synthesis and huwentoxin-I and its structure and bioactivity and analysis. Science in China 40,5.

Bilan Mo, Jiang Li, Songping Liang. 1997. A method for preparation of amino acid thiohydantoins from free amino acids activated by acetyl chloride for development of protein C-terminal sequencing. Analytical Biochemistry 249(2), 207-211.

Song-Ping Liang and Xin Pan (1995) A Lectin-Like Peptide Isolated from the venom of the Chinese bird spider Selenocosmia Huwena Toxicon. Vol. 33. No. 7. pp. 875-882

Song-Ping Liang, Dong-Yi Zhang, Xin Pan, Qun Chen and Pei-Ai Zhou (1993) Properties and Amino acid Sequence of Huwentoxin-I, A neurotoxin purified from the venom of the Chinese Brid Spider Selenocosmia Huwena Toxicon, Vol. 31, No. 8: 969-978

Zhang, D.; Liang, S. 1993. Assignment of the three disulfide bridges of huwentoxin-I, a neurotoxin from the spider selenocosmia huwena. J Protein Chem 12, (6), 735-40.
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